Humaan SORD antilichaam

SORD, also known as sorbitol dehydrogenase, is a 357 amino acid protein of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney, lens of the eye and malignant prostate tissue. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. SORD deficiency may be associated with diabetic complications such as cataracts and microvascular problems.

Sorbitol dehydrogenase, Sorbitol dehydrogenase L iditol 2 dehydrogenase, SDH, SORD, SORD 1, SORD1

Human

Human

Recombinant human SORD (1-357aa) purified from E. coli

AT10F4

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

SORD, ATGA0283-10 µg, ATGA0283-20 µg, ATGA0283-50 µg, ATGA0283-100 µg, ATGA0283-250 µg, ATGA0283-500 µg, ATGA0283-1 mg, ATGA0283-10, ATGA0283-20, ATGA0283-50, ATGA0283-100, ATGA0283-250, ATGA0283-500, ATGA0283-1

Frenette. G., et al. (2006) J Androl 27: 233-239. ; ; Szabo. Z., et al. (2010) Oncol Rep 23: 1233-1239.; ; Lanaspa. M.A., et al. (2009) J Biol Chem 284: 19974-19981.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

AAH25295

Q00796

The recombinant protein (20ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SORD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: SORD recombinant protein ; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: HeLa cell lysate;

ICC/IF analysis of SORD in Hep3B cells. The cell was stained with ATGA0283 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .