Humaan SSU72 antilichaam

SSU72 RNA polymerase II CTD phosphatase homolog, also known as SSU72, is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/ termination machinery. Existing as multiple alternatively spliced isoforms, SSU72 interacts with TFIIB, Rb and DNAM-1 and functions to catalyze the dephosphorylation of target proteins, possibly playing a role in RNA processing and termination via dephosphorylation of Pol II.

SSu72 RNA polymerase II CTD phosphatase homolog, HSPC182, PNAS-120

Human

Human

Recombinant human SSU72 (1-194aa) purified from E. coli

AT66D10

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

SSU72, ATGA0469-10 µg, ATGA0469-20 µg, ATGA0469-50 µg, ATGA0469-100 µg, ATGA0469-250 µg, ATGA0469-500 µg, ATGA0469-1 mg, ATGA0469-10, ATGA0469-20, ATGA0469-50, ATGA0469-100, ATGA0469-250, ATGA0469-500, ATGA0469-1

Dichtl B., et al. (2002) Mol Cell. 10: 1139-1150.; ; Krishnamurthy S., et al. (2004) Mol Cell. 14: 387-394.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_054907

Q9NP77

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSU72 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: MCF-7 cell lysate; ; Lane 7.: SK-OV-3 cell lysate; ; Lane 8.: LnCap cell lysate; ; Lane 9.: HeLa cell lysate