Humaan STEAP1-antilichaam

Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewings sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.

Six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24

Human

Human

Recombinant human STEAP1 (1-70aa) purified from E. coli

J2D2

Unconjugated

ELISA, WB, ICC/IF, IHC

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

STEAP1, AST0910-10 µg, AST0910-20 µg, AST0910-50 µg, AST0910-100 µg, AST0910-250 µg, AST0910-500 µg, AST0910-1 mg, AST0910-10, AST0910-20, AST0910-50, AST0910-100, AST0910-250, AST0910-500, AST0910-1

David A. Rodeberg, et al. (2005) Clin Cancer Res. June 11 (12) : 4545-4552.; ; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12) : 5798-5805.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_036581

Q9UHE8

The cell lysates (30ug) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: LNcap cell lysate; ; Lane 2.: Mouse Testis Tissue lysate;

Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {AST0910-IHC1b.jpg}

ICC/IF analysis of STEAP1 in LNCap cells. The cell was stained with AST0910 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .