Humaan SUMO 2/3 antilichaam

SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8-11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of a variety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residues after the GG region of these precursors by SUMO-specific proteases in maturation is a prerequisite for subsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recent studies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.

Ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, SuMO3, SuMO2, SMT3H2, SMT3H1, SMT3B, SMT3A, SMT3 suppressor of mif two 3 homolog 2, SMT3 homolog 2, SMT 3B, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), Small ubiquitin related modifier 2, Small ubiquitin like modifier 2, Sentrin2, MGC117191, HSMT3

Human

Human

Recombinant human SUMO2 (1-93aa) purified from E.coli

AT10F1

Unconjugated

ELISA, WB, ICC/IF, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

HSMT 3, HSMT3, MGC117191, Sentrin 2, Sentrin2, Sentrin-2, Small ubiquitin like modifier 2, Small ubiquitin related modifier 2, Small ubiquitin-related modifier 2, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), SMT 3B, SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 2, SMT3A, SMT3B, SMT3H1, SMT3H2, SuMO 3, SuMO2, SuMO-2, SuMO3, SuMO-3, ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, ATGA0184-10 µg, ATGA0184-20 µg, ATGA0184-50 µg, ATGA0184-100 µg, ATGA0184-250 µg, ATGA0184-500 µg, ATGA0184-1 mg, ATGA0184-010, ATGA0184-020, ATGA0184-050, ATGA0184-100, ATGA0184-250, ATGA0184-500, ATGA0184-01M

Tatsuya Ii, et al. (2007) DNA Repair (Amst), 6 (11) : 1679-1691.; ; Zheng Xu, et al. (2005) Biochem J, 386 (Pt 2) : 325-330.; ; Melchior F. (2000) Annu Rev Cell Dev Biol, 16: 591-626.

Ham JH, et al. An Immunohistochemical Study on the Expression of SUMO-2/3 in the Colorectal Carcinoma. (Soonchunhyang Med Sci. 2012) {https://jsms.sch.ac.kr/journal/view.php?year=2012&vol=18&no=2&spage=95}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_008868

P61956

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: K562 cell lysateThe cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HL-60 cell lysate; ; {ATGA0184-WB2.jpg}The recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human SUMO1 protein; ; Lane 2.: Recombinant human SUMO2 protein; ; Lane 3.: Recombinant human SUMO3 protein; ; {ATGA0184-WB3.jpg}

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .

Flow cytometry analysis of SUMO2/3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red) .