Humaan Synucleïne-alfa/bèta-antistoffen

Alpha-synuclein (amino acids 119-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-Abeta component of amyloid plaques is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.

SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

Human

Human

Recombinant human a-synuclein (119-140aa) purified from E. coli

3B6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Synuclein beta, Synuclein alpha, SNCB protein, SNCB, SNCA protein, SNCA, PD1, Parkinson disease familial 1, PARK4, PARK1, Non A4 component of amyloid precursor, Non A beta component of AD amyloid, NACP, beta synuclein, alpha synuclein, a/b-Synuclein, a, b-Synuclein, a b-Synuclein, ASC0632-10 µg, ASC0632-20 µg, ASC0632-50 µg, ASC0632-100 µg, ASC0632-250 µg, ASC0632-500 µg, ASC0632-1 mg, ASC0632-010, ASC0632-020, ASC0632-050, ASC0632-100, ASC0632-250, ASC0632-500, ASC0632-01M

Jakes R., et al. (1994) FEBS lett. 345, 27-32.; ; Ueda K., et al. (1993) Proc. Natl.Acad. Sci. USA 90, 11282-11286.; ; Kim J. (1997) Molecules and Cells 7, 78-83.; ; Paik S. R., et al. (1997) Arch. Biochem. Biophys. 344, 325-334.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_000336, NP_003076

P37840, Q16143

The recombinant protein (20ng) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human alpha-synuclein protein; ; Lane 2.: Recombinant human beta-synuclein protein; ; Lane 3.: Recombinant human gamma-synuclein protein; ; Lane 4.: Mouse brain Tissue lysate; ; Lane 5.: Rat brain Tissue lysate;

Flow cytometry analysis of alpha, beta-Synuclein in LNCaP cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ASC0632-FC2.jpg}Flow cytometry analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ASC0632-FC3.jpg}

ICC/IF analysis of alpha, beta-Synuclein in LNCaP cells. The cell was stained with ACS0632 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . ICC/IF analysis of alpha, beta-Synuclein in U87MG cells. The cell was stained with ACS0632 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ASC0632-IF2.jpg}ICC/IF analysis of alpha, beta-Synuclein in C6 cells. The cell was stained with ACS0632 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ASC0632-IF3.jpg}