Humaan TBCA antilichaam

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.

Tubulin folding cofactor A, Tubulin folding cofactor A, chaperonin cofactor a, tubulin specific chaperone a, TBCA, Tubulin folding cofactor A CFA, Co chaperonin associated with a & b tubulin, Cofactor A, TCP1 chaperonin cofactor A, Tubulin cofactor a

Human

Human

Recombinant TBCA (1-108aa) purified from E. coli

AT1A5

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

TBCA, ATGA0155-10 µg, ATGA0155-20 µg, ATGA0155-50 µg, ATGA0155-100 µg, ATGA0155-250 µg, ATGA0155-500 µg, ATGA0155-1 mg, ATGA0155-10, ATGA0155-20, ATGA0155-50, ATGA0155-100, ATGA0155-250, ATGA0155-500, ATGA0155-1

Nolasco S, et al., (2005) FEBS Lett 579 (17) :3515-3524.; ; Tian G, et al., (1996) Cell 86 (2) :287-296.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cell Cycle

NP_004598

O75347

The cell lysate of LNCap (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate; ; {ATGA0155-WB2.jpg}

Flow cytometry analysis of TBCA in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

ICC/IF analysis of TBCA in A549 cells. The cell was stained with ATGA0155 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of TBCA in HeLa cells. The cell was stained with ATGA0155 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010129463677.jpg}