Humaan TBCB antilichaam

Tubulin folding cofactor B, also known as TBCB, belongs to the Microtubules family. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth.

Tubulin folding cofactor B, CG22, CKAP1, CKAPI

Human

Human

Recombinant human TBCB (1-244aa) purified from E. coli

AT1F6

Unconjugated

ELISA, WB, FACS, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

TBCB, Tubulin folding cofactor B, CG22, CKAP1, CKAPI, ATGA0302-10 µg, ATGA0302-20 µg, ATGA0302-50 µg, ATGA0302-100 µg, ATGA0302-250 µg, ATGA0302-500 µg, ATGA0302-1 mg, ATGA0302-10, ATGA0302-20, ATGA0302-50, ATGA0302-100, ATGA0302-250, ATGA0302-500, ATGA0302-1

Kortazar D., et al. (2007) Exp Cell Res. 313:425-436.; ; Tian G., et al. (1997) J Cell Biol. 138: 821-832; ; Fanarraga ML., et al. (2009) Exp cell Res 315:535-541;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1500

Cytoskeleton

NP_001272

Q99426

The cell lysates of Jurkat (40ug) and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCB antibody (1:1500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry analysis of TBCB in HeLa cells. The cell was stained with ATGA0302 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of TBCB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TBCB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .