Humaan TIP30/HTATIP2-antilichaam

HTATIP2, also known as TIP30, is a member of the short-chain dehydrogenases/reductases (SDR) family. It acts as a tumor suppressor participating in metabolic suppression, inhibition of angiogenesis and induces the expression of apoptosis related genes Bad and Siva. HTATIP2 interacts with the activation domain of HIV-1 TAT and enhances its transcription by phosphorylating RNA polymerase II (Pol II) . Defects in This protein are associated with hepatocellular carcinomas and apoptotic resistant tumor cells, suggesting a potential use for HTATIP2 in antitumor therapy.

Oxidoreductase HTATP2, CC3, TIP30, SDR44u1, Oxidoreductase HTATP2, HTATIP 2, HTATIP-2, CC-3, TIP 30, TIP-30

Human

Human

Recombinant human HTATIP2 (1-242aa) purified from E. coli

AT5D6

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

TIP-30, TIP30, TIP 30, SDR44u1, Oxidoreductase HTATP2, HTATIP-2, HTATIP2, HTATIP 2, CC-3, CC3, CC 3, ATGA0444-10 µg, ATGA0444-20 µg, ATGA0444-50 µg, ATGA0444-100 µg, ATGA0444-250 µg, ATGA0444-500 µg, ATGA0444-1 mg, ATGA0444-010, ATGA0444-020, ATGA0444-050, ATGA0444-100, ATGA0444-250, ATGA0444-500, ATGA0444-01M

Shtivelman E., et al. (1997) Oncogene. 14 (18) : 2167-73. ; ; Xiao H., et al. (1998) Proc Natl Acad Sci u S A. 95 (5) : 2146-2151.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

AAH15358

Q9BUP3

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HTATIP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HeLa cell lysate

ICC/IF analysis of HTATIP2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HTATIP2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of HTATIP2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HTATIP2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0444-IF2.jpg}