Humaan TLR7 antilichaam

TLR7 is one of toll-like receptors which are type I transmembrane proteins involved in innate immunity byrecognizing microbial conserved structures. These proteins are characterized by multiple copies of leucine richrepeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain, and act through adaptormolecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Human TLR7 (hTLR7) is predominantly expressed in lung, placenta and spleen and hTLR7, 8 genes are located on the sexchromosome X. hTLR7 may play an important role in detecting and combating viral infections.

Toll-like receptor 7, PRO285, UNQ248, tlr 7

Human

Human

Recombinant human TLR7 (451-500aa) purified from E. coli.

4F4

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

TLR7, ATGA0530-10 µg, ATGA0530-20 µg, ATGA0530-50 µg, ATGA0530-100 µg, ATGA0530-250 µg, ATGA0530-500 µg, ATGA0530-1 mg, ATGA0530-10, ATGA0530-20, ATGA0530-50, ATGA0530-100, ATGA0530-250, ATGA0530-500, ATGA0530-1

Lee J., et al. (2003) Proc. Natl. Acad. Sci. USA. 100:6646-6651.; ; Crozat K., et al. (2004) Proc. Natl. Acad. Sci. USA. 101:6835-6836.; ; Muzio M., et al. (1998) J. Exp. Med. 187:2097-2101.; ; Horng T., et al. (2002) Nature 420:329-333.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenetics and Nuclear Signaling

NP_057646

Q9NYK1

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TLR7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human TLR7 protein;

Flow cytometry analysis of TLR7 in THP-1 cells. The cell was stained with ATGA0530 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .