Humaan Tollip antilichaam

Toll interacting protein, also known as Tollip, is an inhibitory adaptor protein within the signaling pathwayof IL-1 and Toll-like receptors (TLR) . The Toll-like receptors are essential transmembrane signalingreceptors of the innate immune system that alert the host to the presence of a microbial invader. Tolliprecruits IRAk1 to the IL-1 receptor complex and inhibits IRAk1 phosphorylation and kinase activity.

Toll interacting protein, IL-1RAcPIP

Human

Human

Recombinant human Tollip (61-274aa) purified from E. coli

K1B17

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Tollip, ATGA0568-10 µg, ATGA0568-20 µg, ATGA0568-50 µg, ATGA0568-100 µg, ATGA0568-250 µg, ATGA0568-500 µg, ATGA0568-1 mg, ATGA0568-10, ATGA0568-20, ATGA0568-50, ATGA0568-100, ATGA0568-250, ATGA0568-500, ATGA0568-1

Ciarrocchi A. et al., (2009) Published online. 4 (2) :e4404.; ; Didierlaurent A. et al., (2006) Mol Cell Biol. 26 (3) :735-742.; ; Opal S.M. et al., (2002) Crit Care. 6 (2) :125-136.; ; Burns K. et al., (2000) Nat Cell Biol. 2 (6) :346-351.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_061882

Q9H0E2

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tollip antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HepG2 cell lysate;