Humaan TREM2-antilichaam

The triggering receptor expressed on myeloid cells-2 (TREM2) is a membrane protein that interacts with the TYROBP protein. The TREM2 and TYROBP proteins form a complex that transmits chemical signals to activate the cell. This complex is involved in the growth and development of several types of immune cells, particularly dendritic cells and activates these cells, triggering an inflammatory response to injury or disease. And the TYROBP-TREM2 complex also activates cells in the skeletal system and in the brain and spinal cord (central nervous system) .

Ttriggering receptor expressed on myeloid cells 2 isoform 1, Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on myeloid cells 2a, TREM-2, Trem2a, Trem2b, Trem2c

Human

Human

Recombinant human TREM2 (19-161aa) purified from E. coli

AT1E7

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ATGA0605-10 µg, ATGA0605-20 µg, ATGA0605-50 µg, ATGA0605-100 µg, ATGA0605-250 µg, ATGA0605-500 µg, ATGA0605-1 mg, ATGA0605-010, ATGA0605-020, ATGA0605-050, ATGA0605-100, ATGA0605-250, ATGA0605-500, ATGA0605-01M, ATR0917-10 µg, ATR0917-20 µg, ATR0917-50 µg, ATR0917-100 µg, ATR0917-250 µg, ATR0917-500 µg, ATR0917-1 mg, ATR0917-010, ATR0917-020, ATR0917-050, ATR0917-100, ATR0917-250, ATR0917-500, ATR0917-01M

N.Diaye EN, et al., (2009) J Cell Biol. 184 (2) :215-23.; ; Piccio L, et al., (2008) Brain. 131 (11) :3081-91.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology, Neuroscience

NP_061838

Q9NZC2

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : TREM2 19-161aa Transfected 293T cell lysate;

Flow cytometry analysis of TREM2 in Hep3B cells. The cell was stained with ATGA0605 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of TREM2 in HepG2 cells. The cell was stained with ATGA0605 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of TREM2 in Raw264.7 cells. The cell was stained with ATGA0605 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2501170001612095.jpg}