Humaan Troponine I1/TNNI1-antilichaam

Troponin I, slow skeletal muscle, also known as TNNI1, belongs to the troponin I family. The troponin I subfamily contains three genes: TNNI-skeletal-fast-twitch, TNNI-skeletal-slow-twitch, and TNNI-cardiac. The TNNI-fast and TNNI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TNNI-cardiac gene is expressed exclusively in cardiac muscle tissue. TNNI1 is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation.

Troponin I1 slow skeletal muscle, Troponin I, slow skeletal muscle, DKFZp451O223, SSTNI, TNN1

Human

Human

Recombinant human TNNI1 (1-187aa) purified from E. coli

AT36E7

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

TNNI1, ATGA0558-10 µg, ATGA0558-20 µg, ATGA0558-50 µg, ATGA0558-100 µg, ATGA0558-250 µg, ATGA0558-500 µg, ATGA0558-1 mg, ATGA0558-10, ATGA0558-20, ATGA0558-50, ATGA0558-100, ATGA0558-250, ATGA0558-500, ATGA0558-1

Hunkeler NM, Kullman J, Murphy AM (1991) . Circ. Res. 69 (5) : 1409-14.; ; Bhavsar PK, Dhoot GK, Cumming DV, et al. (1992) . FEBS Lett. 292 (1-2) : 5-8.; ; Westfall MV, Borton AR (2003) . J. Biol. Chem. 278 (36) : 33694-700.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_003272

P19237

The extracts of mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNNI1 antibody (1:500~5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: TNNI1 mAb 1:500; ; Lane 2.: TNNI1 mAb 1:1000; ; Lane 3.: TNNI1 mAb 1:5000