Humaan UDP-glucosedehydrogenase/UGDH antilichaam

UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH

Human

Human

Recombinant human UGDH (1-494aa) purified from E. coli

AT2G11

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

UGDH, uDP-glucose dehydrogenase, GDH, uDP-GlcDH, uDPGDH, uGD, uDP-glucose dehydrogenase, uDP-glucose 6-dehydrogenase, ATGA0425-10 µg, ATGA0425-20 µg, ATGA0425-50 µg, ATGA0425-100 µg, ATGA0425-250 µg, ATGA0425-500 µg, ATGA0425-1 mg, ATGA0425-10, ATGA0425-20, ATGA0425-50, ATGA0425-100, ATGA0425-250, ATGA0425-500, ATGA0425-1

Bontemps Y., et al. (2003) J Biol Chem. 278 (24) : 21566-75.; ; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70 (2) : 401-10.; ; Egger S., et al. (2011) J Biol Chem. 286 (27) : 23877-87.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_003350

O60701

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UGDH antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: NIH-3T3 cell lysate ; ; Lane 3.: HepG2 cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: MCF7 cell lysate

ICC/IF analysis of UGDH in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UGDH antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0425-IF1b.jpg}