Humaan VAMP-2 antilichaam

Synaptobrevin 2 (Vesicle-associated membrane, VAMP2), which is an 18 kDa integral membrane proteinlocalized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highlyconserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal tail. Synaptobrevin 2 ispredominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms aspecific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.This antibody recognizes specifically synaptobrevin 2, but it also shows a low affinity to synaptobrevin1.

Vesicle-associated membrane protein 2, VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2, Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460, RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associatedmembrane protein 2 (synaptobrevin 2)

Human

Human

Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli

300000

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

VAMP2, ATGA0588-10 µg, ATGA0588-20 µg, ATGA0588-50 µg, ATGA0588-100 µg, ATGA0588-250 µg, ATGA0588-500 µg, ATGA0588-1 mg, ATGA0588-10, ATGA0588-20, ATGA0588-50, ATGA0588-100, ATGA0588-250, ATGA0588-500, ATGA0588-1

Andres C et al., (1995) Proc Natl Acad Sci USA 92:5987-5991.; ; Jacobsson G et al., (1998) European Journal of Neuroscience 16:301-316.; ; Scales SJ. et al (2002) J Biol. Chem. 227 (31) :28271-28279.; ; Pennuto M et al., (2003) Mol Biol Cell. 14:4909-19.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_055047

P63027

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human VAMP1 protein; ; Lane 2.: Recombinant human VAMP2 protein; ; Lane 3.: Recombinant human VAMP3 protein; ; The tissue lysate (10 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain Tissue lysate{ATGA0588-WB2.jpg}

ICC/IF analysis of VAMP-2 in U87MG cells. The cell was stained with ATGA0588 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .