Humaan VHL antilichaam

Von Hippel-Lindau disease (VHL) is a dominant inherited syndrome characterized by the predisposition to develop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas and hemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in the VHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-type allele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich in beta sheet (beta-domain) and a smaller alpha-helical domain (alpha-domain), held together by two linkers and a polar interface. VHL protein is also involved in the degradation of hypoxia-inducible factor (HIF) .

Von Hippel-Lindau tumor suppressor isoform 1, Von Hippel-Lindau tumor suppressor isoform 1, HRCA1, RCA1, VHL1, Von Hippel-Lindau tumor suppressor isoform 1 pVHL, G7 protein, Elongin binding protein, HRCA 1, RCA 1, VHL, VHL 1, VHLH, Von Hippel Lindau disease tumor suppressor, von Hippel Lindau syndrome, von Hippel Lindau tumor suppressor, AVH0616

Human

Human

Recombinant human VHL (1-154aa) purified from E. coli

AT82B10

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

VHL, ATGA0492-10 µg, ATGA0492-20 µg, ATGA0492-50 µg, ATGA0492-100 µg, ATGA0492-250 µg, ATGA0492-500 µg, ATGA0492-1 mg, ATGA0492-10, ATGA0492-20, ATGA0492-50, ATGA0492-100, ATGA0492-250, ATGA0492-500, ATGA0492-1

Latif F., et al. (1993) Science. 260 (5112) : 1317-20.; ; Duan DR., et al. (1995) PNAS. 92 (14) : 6459-63.; ; Maxwell PH., et al. (1999) Nature. 399 (6733) : 271-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_000542

P40337

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Raji cell lysate; ; Lane 4.: Jurkat cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: MCF7 cell lysate; ; Lane 7.: PC3 cell lysate;

ICC/IF analysis of VHL in Hep3B. The cell was stained with ATGA0492 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .