Humaan VHR/DUSP3 antilichaam

DUSP3, also known as Dual specificity protein phosphatase 3, is inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.

Dual specificity protein phosphatase 3, VHR, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, Vaccinia virus phosphatase VH1-related

Human

Human

Recombinant human DUSP3 (1-185aa) purified from E. coli

AT9E6

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

VHR, ATGA0374-10 µg, ATGA0374-20 µg, ATGA0374-50 µg, ATGA0374-100 µg, ATGA0374-250 µg, ATGA0374-500 µg, ATGA0374-1 mg, ATGA0374-10, ATGA0374-20, ATGA0374-50, ATGA0374-100, ATGA0374-250, ATGA0374-500, ATGA0374-1

Sun H. (1998) Mol Biol. 84: 307-318.; ; Folander K., et al. (1995) Genomics. 23 (1) : 295-296.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_004081

P51452

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHR antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: 293T cell lysate; ; Lane 6.: U87MG cell lysate; ; Lane 7.: K562 cell lysate