Humaan vimentine antilichaam

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastaticprogression.

Vimentin, epididymis luminal protein 113, HEL113, CTRCT30

Human

Human

Recombinant human VIM (1-466aa) purified from E. coli

AT5H8

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Vimentin, Vimentin, epididymis luminal protein 113, HEL113, CTRCT30, ATGA0453-10 µg, ATGA0453-20 µg, ATGA0453-50 µg, ATGA0453-100 µg, ATGA0453-250 µg, ATGA0453-500 µg, ATGA0453-1 mg, ATGA0453-10, ATGA0453-20, ATGA0453-50, ATGA0453-100, ATGA0453-250, ATGA0453-500, ATGA0453-1

John E. Eriksson., et al. (2009) J Clin Invest. 119 (7) : 1763-1771. ; ; Cabeen MT., et al. (2010) Annu Rev Genet. 44: 365-392.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_003371

P08670

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Jurkat cell lysate ; ; Lane 2 : K562 cell lysate ; ; Lane 3 : 293T cell lysate ; ; Lane 4 : A549 cell lysate ; ; Lane 5 : SK-OV-3 cell lysate ; ; Lane 6 : HeLa cell lysate

ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0453-IF1b.jpg}