Humaan VTA1-antilichaam

The VTA1 protein is involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. This protein is involved in the sorting and down-regulation of EGFR (By similarity) and involved in HIV-1 budding.

Vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, DRG1, LIP5, SBP1

Human

Human

Recombinant human VTA1 (1-307aa) purified from E. coli

AT14G10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

VTA1, ATGA0403-10 µg, ATGA0403-20 µg, ATGA0403-50 µg, ATGA0403-100 µg, ATGA0403-250 µg, ATGA0403-500 µg, ATGA0403-1 mg, ATGA0403-10, ATGA0403-20, ATGA0403-50, ATGA0403-100, ATGA0403-250, ATGA0403-500, ATGA0403-1

Jun Shi., et al. (2001) Brain Res. 910 (1-2) : 29-37. ; ; Fujita H., et al. (2004) J Cell Sci. 117 (Pt 14) : 2997-3009.; ; Ward DM., et al. (2005) J Biol Chem. 280 (11) : 10548-55.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Virus

NP_057569

Q9NP79

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: Jurkat cell lysate; ; Lane 7.: K562 cell lysate; ; Lane 8.: LnCaP cell lysate; ; Lane 9.: U937 cell lysate; ; Lane 10.: A431 cell lysate

Flow cytometry analysis of VTA1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of VTA1 in Hep3B cells. The cell was stained with ATGA0403 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .