Humaan Wnt-3a antilichaam

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors.

Wnt3a, Wingless-type MMTV integration site family, member 3A

Human

Human

Recombinant human Wnt3a (19-352aa) purified from E. coli

3A6

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Wnt3a, ATGA0520-10 µg, ATGA0520-20 µg, ATGA0520-50 µg, ATGA0520-100 µg, ATGA0520-250 µg, ATGA0520-500 µg, ATGA0520-1 mg, ATGA0520-10, ATGA0520-20, ATGA0520-50, ATGA0520-100, ATGA0520-250, ATGA0520-500, ATGA0520-1

Pan W., et al. (2008) Science. 321 (5894) :1350-1353; ; Katoh M., et al. (2002) Int J Oncol. 20 (2) :373-377; ; Smolich B D., et al. (1993) Mol Biol Cell. 4 (12) :1267-1275

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Development

NP_149122

P56704

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Wnt3a antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Wnt3a transfected 293T cell lysate