Humaan XPA antilichaam

XPA, also known as DNA repair protein complementing XP-A cells, belong to the XPA family. This protein is involved in DNA excision repair. It Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A), which is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.

DNA repair protein complementing XP-A cells, XP1, XPAC

Human

Human

Recombinant human XPA (1-273aa) purified from E. coli

AT71H3

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

XPA, ATGA0502-10 µg, ATGA0502-20 µg, ATGA0502-50 µg, ATGA0502-100 µg, ATGA0502-250 µg, ATGA0502-500 µg, ATGA0502-1 mg, ATGA0502-10, ATGA0502-20, ATGA0502-50, ATGA0502-100, ATGA0502-250, ATGA0502-500, ATGA0502-1

Pan Y.R., et al. (2009) Cell Cycle 8:655-664.; ; James, et al. (2005) Andrews Diseases of the Skin: Clinical Dermatology. (10th ed.) . Saunders.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_000371

P23025

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: SW480 cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: MCF7 cell lysate ; ; Lane 4.: LNCaP cell lysate; ; Lane 5.: Raji cell lysate

ICC/IF analysis of XPA in HaLa cells. The cell was stained with ATGA0502 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .