Human AIF/AIFM1-antilichaam

Apoptosis-inducing factor 1, also known as AIFM1, is a mitochondrial protein that translocates to the nucleus upon induction of apoptosis. AIFM1 has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIFM1 from mitochondria, but not to block its apoptogenic activity. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.

Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, AIF, CMTX4, COWCK, COXPD6, PDCD8

Human

Human

Recombinant human AIFM1 (98-609aa) purified from E. coli

AT22E9

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

AIF, AIFM1, Apoptosis-inducing factor 1, mitochondrial isoform 2 precursor, Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, CMTX4, COWCK, COXPD6, PDCD8, ATGA0411-10 µg, ATGA0411-20 µg, ATGA0411-50 µg, ATGA0411-100 µg, ATGA0411-250 µg, ATGA0411-500 µg, ATGA0411-1 mg, ATGA0411-010, ATGA0411-020, ATGA0411-050, ATGA0411-100, ATGA0411-250, ATGA0411-500, ATGA0411-01M

Tang Y., et al. (2013) J Proteomics. 91C: 200-209.; ; Kim TW., et al. (2013) Cell Death Dis. 4: e919.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_665811

O95831

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Jurkat cell lysate; ; Lane 2. : HeLa cell lysate; ; Lane 3. : Hep3B cell lysate; ; Lane 4. : Raji cell lysate; ; Lane 5. : K562 cell lysate; ; Lane 6. : MCF7 cell lysate; ; Lane 7. : CTLL2 cell lysate The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Mouse heart tissue lysate; ; Lane 2. : Mouse liver tissue lysate {ATGA0411-WB2.jpg}

ICC/IF analysis of AIFM1 in Hep3B cells. The cell was stained with ATGA0411 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .