Human Annexin A1/ANXA1-antilichaam

Annexin A1, also known as Lipocortin I, belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. It promotes membrane fusion and is also involved in exocytosis. Since Annexin A1 has phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity, this protein may have potential anti-inflammatory activity. The detection of this protein by immunocytochemical means reportedly provides a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia.

Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, phospholipase A2 inhibitory protein, p35, Annexin Ac2-26, ANX1, LPC1

Human

Human

Recombinant human Annexin A1 (1-346aa) purified from E.coli

AT2G5

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Phospholipase A2 inhibitory protein, p35, LPC-1, LPC1, LPC 1, Lipocortin-I, LipocortinI, Lipocortin-1, Lipocortin1, Lipocortin I, Lipocortin 1, HGNC:533, Chromobindin-9, Chromobindin9, Chromobindin 9, Calpactin-II, CalpactinII, Calpactin-2, Calpactin2, Calpactin II, Calpactin 2, ANXA1 protein, ANXA1, ANXA 1, ANX1, ANX A1, ANX 1, Annexin-I, AnnexinI, AnnexinA1, Annexin-1, Annexin1, Annexin I, Annexin Ac2-26, Annexin Ac2 26, Annexin A1, Annexin 1, ATGA0336-10 µg, ATGA0336-20 µg, ATGA0336-50 µg, ATGA0336-100 µg, ATGA0336-250 µg, ATGA0336-500 µg, ATGA0336-1 mg, ATGA0336-010, ATGA0336-020, ATGA0336-050, ATGA0336-100, ATGA0336-250, ATGA0336-500, ATGA0336-01M

Lim L.H., et al. (2007) FASEB J. 21 (4) : 968-975.; ; Kovacic R.T., et al. (1991) Biochemistry. 30 (37) : 9015-9021.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_000691

P04083

The Recombinant protein (10ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human Annexin A1 ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: A549 cell lysate ; ; Lane 4.: A431 cell lysate The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Anti- Annexin A1 antibody (1:10000) Lane 2.: Anti- Annexin A1 antibody (1:20000) {ATGA0336-WB2.jpg}