Humane aldehydededehydrogenase 2/ALDH2-antilichaam

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations.

Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM

Human

Human

Recombinant human ALDH2 (18-517aa) purified from E. coli

AT2C10

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ALDH2, Aldehyde dehydrogenase mitochondrial, ALDH-E2, ALDHI, ALDM, Aldehyde dehydrogenase, Mitochondrial Acetaldehyde dehydrogenase 2, Aldehyde dehydrogenase 2 family, Aldehyde dehydrogenase 2 family (mitochondrial), ALDHE2, ALDH 2, ALDH class 2, ALDH E2, Liver mitochondrial ALDH, MGC1806, Mitochondrial aldehyde dehydrogenase 2, Nucleus encoded mitochondrial aldehyde dehydrogenase 2, ATGA0450-10 µg, ATGA0450-20 µg, ATGA0450-50 µg, ATGA0450-100 µg, ATGA0450-250 µg, ATGA0450-500 µg, ATGA0450-1 mg, ATGA0450-010, ATGA0450-020, ATGA0450-050, ATGA0450-100, ATGA0450-250, ATGA0450-500, ATGA0450-01M

Crabb DW., et al. (1989) J Clin Invest. 83 (1) : 314-6. ; ; Feng Liu., et al. (2002) Plant Physiol. 130 (4) : 1657-1674.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Redox Proteins

NP_000681

P05091

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HepG2 cell lysate ; ; Lane 2. : Mouse liver tissue lysate ; ; Lane 3. : Mouse lung tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate ; ; Lane 2. : ALDH2 transfected 293T cell lysate {ATGA0450-WB2.jpg}

ICC/IF analysis of ALDH2 in A549 cells line, stained with monoclonal anti-human ALDH2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .