Humane anorganische pyrofosfatase/PPA1-antilichaam

PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation.

Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

Human

Human

Recombinant human PPA1 (1-289aa) purified from E. coli

AT4G4

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Inorganic Pyrophosphatase, PPA1, Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061, ATGA0433-10 µg, ATGA0433-20 µg, ATGA0433-50 µg, ATGA0433-100 µg, ATGA0433-250 µg, ATGA0433-500 µg, ATGA0433-1 mg, ATGA0433-10, ATGA0433-20, ATGA0433-50, ATGA0433-100, ATGA0433-250, ATGA0433-500, ATGA0433-1

Carman GM, et al., (2006) Trends Biochem. Sci. 31 (12) : 694-9. ; ; Usui Y, et al., (2010) J. Dent. Res. 89 (5) : 504-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_066952

Q15181

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : A431 cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4. : HepG2 cell lysate ; ; Lane 5. : U87MG cell lysate ; ; Lane 6. : PC3 cell lysate ; ; Lane 7. : WiDr cell lysate

Flow cytometry analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .