Humane bèta-NGF snelle ELISA-test

FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.

The Biosensis NGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of human NGF in less than 3 hours in cell culture supernatants, serum, plasma (citrate) and brain extracts and thus employs a recombinant human NGF standard approved by the World Health Organization (WHO) . Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from numerous other species including mouse and rat.

Beta-nerve growth factor; Ngfb; NGF

P01138

Human

Recombinant human NGF, made in CHO cells (WHO reference reagent)

Nerve growth factor (Beta-NGF)

ELISA

Culture Supernatant, Plasma (Citrate), Serum, Tissue Homogenates

For research use only.

Shipped on ice packs

Store at 2-8°C

The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, human proNGF protein shows <0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer), suggesting the preferential quantification of mature NGF over full-length human proNGF. This NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect mature NGF from numerous other species including mouse and rat.

Colorimetric

Human