Humane CISD1-antilichaam

CISD1 (CDGSH iron-sulfur domain-containing protein 1) is 108 amino acid protein. CISD1 plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions. This protein expression is reduced in cells derived from cystic fibrosis patients.

DGSH iron-sulfur domain-containing protein 1, Cysteine transaminase CISD1

Human

Human

Recombinant human CISD1 (32-108aa) purified from E. coli

AT1A8

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CISD1, ATGA0242-10 µg, ATGA0242-20 µg, ATGA0242-50 µg, ATGA0242-100 µg, ATGA0242-250 µg, ATGA0242-500 µg, ATGA0242-1 mg, ATGA0242-10, ATGA0242-20, ATGA0242-50, ATGA0242-100, ATGA0242-250, ATGA0242-500, ATGA0242-1

Wiley, S.E., et al. (2007) J Biol Chem 282: 23745-23749.; ; Paddock, M.L., et al. (2007) Proc Natl Acad Sci USA 104: 14342-14347.; ; Taminelli, G.L., et al. (2008) Biochem Biophys Res Commun 365: 856-862.

He QQ, et al. MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection. (Sci Rep. 2016) {https://pubmed.ncbi.nlm.nih.gov/27748416/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_060934

Q9NZ45

The tissue lysate of Mouse liver (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: MCF7 cell lysate {ATGA0242-WB2.jpg}

Flow cytometry analysis of CISD1 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .