Humane IL-35 (Interleukin 35) ELISA Kit

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IL-35 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-35 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-35 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-35 can be calculated using the standard curve.

ELISA

1 x 96 well Format (96 Tests)

Please refer to the Manual

https://www.abeomics.com/images/90-2246/Human%20IL-35_Manual.pdf

This immunoassay kit allows for the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma and other biological fluids. Detection Range: 15.6 - 1000 pg/ml Sensitivity: < 9.4 pg/ml