Humane IL-4 (Interleukin 4) ELISA Kit met pre-Coated

This assay employs an antibody specific for Anti-human IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

ELISA

1 x 96 well Format (96 Tests)

Please refer to the Manual

https://www.abeomics.com/images/90-2092/Human%20IL-4_Manual.pdf

This immunoassay kit allows for the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids. Detection Range: 31.2 - 2000 pg/ml Sensitivity: < 18.75 pg/ml