HUS1 cDNA

HuS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1 (RAD9-RAD1-HuS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response

HHUS1

7p13-p12

Human

PATGen (puc19-derived cloning vector)

ATGAAGTTTC GGGCCAAGAT CGTGGACGGG GCCTGTCTGA ACCACTTCAC ACGAATCAGT AACATGATAG CCAAGCTTGC CAAAACCTGC ACCCTCCGCA TCAGCCCTGA TAAGCTTAAC TTCATCCTTT GTGACAAGCT GGCTAATGGA GGAGTGAGCA TGTGGTGTGA GCTGGAACAG GAGAACTTCT TCAACGAATT TCAAATGGAG GGTGTCTCTG CAGAAAACAA TGAGATTTAT TTAGAGCTAA CATCGGAAAA CTTATCTCGA GCCTTGAAGA CTGCCCAGAA TGCCAGGGCT TTGAAAATCA AACTGACTAA TAAACACTTT CCCTGCCTCA CGGTCTCCGT GGAGCTGTTA TCTATGTCAA GCAGTAGCCG CATTGTGACC CATGACATCC CCATAAAGGT GATTCCTAGG AAATTGTGGA AGGACTTACA AGAACCGGTG GTCCCAGATC CTGATGTTAG TATTTATTTA CCAGTCTTGA AGACTATGAA GAGTGTTGTG GAAAAAATGA AAAACATCAG CAATCACCTT GTTATTGAAG CAAACCTAGA TGGAGAATTG AATTTGAAAA TAGAAACTGA ATTAGTATGT GTTACAACTC ATTTTAAAGA TCTTGGAAAT CCTCCATTAG CCTCTGAAAG CACCCATGAG GACAGAAACG TGGAACACAT GGCTGAAGTG CACATAGATA TTAGGAAGCT CCTACAGTTT CTTGCTGGAC AACAAGTAAA TCCCACAAAG GCCTTATGCA ATATTGTGAA TAACAAGATG GTGCATTTTG ATCTGCTTCA TGAAGACGTG TCCCTTCAGT ATTTCATCCC TGCGCTGTCC TAG

HUS1, hHUS1, HUS1, ATGD0082-10 µg, ATGD0082-20 µg, ATGD0082-50 µg, ATGD0082-100 µg, ATGD0082-250 µg, ATGD0082-500 µg, ATGD0082-1 mg, ATGD0082-10, ATGD0082-20, ATGD0082-50, ATGD0082-100, ATGD0082-250, ATGD0082-500, ATGD0082-1

Store the plasmid at -20C.

Lyophilized

Cell Cycle

603760

NP_004498.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_004507.3

843bp

This shuttle vector contains the complete ORF. It is inseted BamH I to EcoR I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.