Hydroxyzuuroxidase 1 Antilichaam / HAO1

Hydroxyacid oxidase (glycolate oxidase) 1 is a protein that in humans is encoded by the HAO1 gene. This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q9UJM8

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids K48-I370) was used as the immunogen for the Hydroxyacid oxidase 1 antibody.

WB, IHC-P, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Hydroxyacid oxidase 1 antibody is available for research use only.

After reconstitution, the Hydroxyacid oxidase 1 Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Hydroxyacid oxidase 1 antibody should be determined by the researcher.

IHC staining of FFPE mouse liver tissue with Hydroxyacid oxidase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.