IARS2 Antilichaam / Isoleucine--tRNA ligase, mitochondriaal

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q9NSE4

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids D702-K1012) was used as the immunogen for the IARS2 antibody.

WB, IHC-P, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This IARS2 antibody is available for research use only.

After reconstitution, the IARS2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the IARS2 antibody should be determined by the researcher.

Cytoplasm (Mitochondria)

IHC staining of FFPE human rectum adenocarcinoma tissue with IARS2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.