ICAD (DFFA) Mouse Monoclonal Antibody [Kloon ID: LBI3F12]

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Carrier-free (BSA/glycerol-free) DFF45 mouse monoclonal antibody, clone LBI3F12

DFF-45; DFF1; ICAD

Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.

1676

O00273

Unconjugated

Apoptosis

Apoptosis

WB 1:200~500, IF 1:100

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

DFFA

https://www.ncbi.nlm.nih.gov/gene/?term=1676

https://www.uniprot.org/uniprot/O00273