ICAM1 Antilichaam / CD54

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

P05362

Mouse

A synthesized peptide derived from human ICAM1 / CD54 was used as the immunogen for the ICAM1 antibody.

32I16

WB, IHC

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This ICAM1 antibody is available for research use only.

Store the ICAM1 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the ICAM1 antibody should be determined by the researcher.

Cell surface

Immunohistochemical staining of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ICAM1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.