IDH3A Antilichaam / isocitraatdehydrogenase [NAD] subeenheid alfa

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (IDH3?) is an enzyme that in humans is encoded by the IDH3A gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+) -dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD (+) -dependent isocitrate dehydrogenase.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P50213

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids K9-D366) was used as the immunogen for the IDH3A antibody.

WB, IHC-P, IF, FACS, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This IDH3A antibody is available for research use only.

After reconstitution, the IDH3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the IDH3A antibody should be determined by the researcher.

Cytoplasm

Immunofluorescent staining of FFPE human liver cancer tissue with IDH3A antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.