IGF1R-eiwit (C-His, mens)

IGF1R

CD221, IGFIR, IGFR, JTK13, MGC18216

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

> 90 % as determined by SDS-PAGE

1.Immobilized Anti-IGF-1R (Research Grade Teprotumumab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human IGF1R / CD221 Protein (His Tag) (Cat: 10164-H08H), the EC50 is 1.2-3.6 ng/mL (QC tested) . 2.Loaded Anti-IGF-1R (Research Grade Teprotumumab Biosimilar) on ProA Biosensor, can bind Recombinant Human IGF1R Protein, His Tag (Cat. No. 10164-H08H) with an affinity constant of 0.808nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Recombinant Human IGF1R / CD221 Protein (His Tag) (Cat: 10164-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human IGF1 Protein (Fc Tag) (Cat: 10598-H01H1) with an affinity constant of 36.400 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 4.Immobilized Recombinant Human IGF1R / CD221 Protein (His Tag) (Cat: 10164-H08H) at 5 μg/mL (100 μL/well) can bind Recombinant Human IGF1 Protein (Fc Tag) (Cat: 10598-H01H1), the EC50 is 85.9 ng/mL (Routinely tested) .

Met1-Asn932

The calculated molecular masses for α subunit, β subunit and the single chain of the receptor are approximately 81 kDa, 23 kDa and 104 kDa, respectively. As a result of glycosylation, the recombinant single chain, α subunit and β subunit have apparent molecular masses of approximately 150 kDa, 120 kDa and 48 kDa, respectively in SDS-PAGE under reducing conditions.

Human