IGFBP3-eiwit (N-His, mens)
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
IGFBP3
BP-53, IBP-3, IBP3, IGFBP-3
• HEK 293
N-His
< 1.0 EU per μg of the protein as determined by the LAL method
> 85 % as determined by SDS-PAGE
1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 (Cat:13032-H24E) at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized human IGF1 (Cat:10598-H24E) at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 24 ng/mL. 3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.) . The ED50 for this effect is typically 0.05-0.2 μg/mL in the presence of 14 ng/mL human IGFII.
Gly28-Lys291
The secreted recombinant human IGFBP3 comprises 280 amino acids with a predicted molecular mass of 31 kDa. As a result of glycosylation, the apparent molecular mass of rhIGFBP3 is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
Human
Beschrijving
IGFBP3-eiwit (N-His, mens) Beschikbaar in 100 µg. Bestel eenvoudig online met snelle levering.
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