IKK epsilon-antilichaam

IKK epsilon Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. Iκ B is phosphorylated by Iκ B kinase (IKK) complex that contains IKKα , IKKβ , and IKKγ . A novel molecule in the IKK complex was recently identified and designated IKKε /IKK-i. IKK epsilon is required for the activation of NF-κ B by PMA and T cell receptors but not by TNFα and IL-1. IKKε /IKK-i message is expressed in a variety of tissues and is inducible by TNFα , IL-1, and LPS.

9641

Q14164

AF241789

Human, Mouse

2329

Unconjugated

Primary Antibodies

Induced Expression Validation (Figure 1) : Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (3 hrs and 6 hrs) . IKK epsilon expression detected by anit-IKK epsilon antibodies (2329) was induced by LPS treatment and the expression levels were increased gradually at 3 hrs and 6 hrs. Induced Expression Validation2329

Signal Transduction

IKK epsilon Antibody is affinity chromatography purified via peptide column.

Cat. No. 1205 - Jurkat Cell Lysate

1 mg/ml

Predicted species reactivity based on immunogen sequence: Rat: (75%)

IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

IKK epsilon antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

It has no cross response to IKKa, IKKb, or IKKy.

Human IKK epsilon has 2 isoforms, including isoform 1 (716aa, 80kD) and isoform 2 (631aa, 70kD) . Mouse IKK epsilon has one isoform (717aa, 81kD) and Rat IKK epsilon also has one isoform (717aa, 80kD) . 2329 can detect human, mouse and possibly rat.

IKBKE

Cat. No. 1307 - Human Pancreas Tissue Lysate

Cat. No. 11-101 - Human Pancreas Tissue Slide

Cat. No. 1202 - A431 Cell Lysate

Cat. No. 1224 - Daudi Cell Lysate

Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon

Homo sapiens

Peters et al. Mol Cell 2000;5 (3) :513-22.

Shimada et al. Int Immunol 1999;11 (8) :1357-62 (WD0101) .

IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon

ELISA, WB, IHC-P, IF

7288877

Liquid