IL-13 (109aa)

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is an 11.9 kDa protein consisting of 109 amino acid residues.

Il13

116553

P42203

NP_446280.1

NM_053828

10q22

Rat

Human, Rat

VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSMVWSVDL TAGGFCAALE SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY SKQLFRYGH

< 0.1 ng/µg of protein (< 1EU/µg)

> 98% by SDS-PAGE & HPLC analyses

The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells is < 5 ng/ml, corresponding to a specific activity of > 2 x 105 units/mg.

109

Lyophilized

11.9 kDa

E. coli