IL-22 gepegyleerd

Mono-pegylated (with 20 kDa PEG) recombinant mouse interleukin 22 is one polypeptide chain containing 147 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 36 kDa as determined by MS. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. For more details see L. Niv-Spector et al. Protein Eng Des Sel. (PEDS) 25:397-404 (2012) .

IL22; IL-22; Iltif; IL-22a; ILTIFa

50929

Q9JJY9

NP_058667.1

NM_016971

Mouse

Mouse

PEG

ALPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV

It is recommended to reconstitute the lyophilized pegylated mouse IL22 in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.

< 0.1 ng/µg of protein (< 1EU/µg)

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Recombinant murine IL-22 is biologically active when compared to standard using STAT3 phosphorylation assay in HepG cells. Its acrtivity in vitro is ~ 10% compared to the non-pegylated mIL22.

147

Lyophilized

Water

36.0 kDa (monomer)

E. coli

ALPVN