IL-27

As a member of the IL-12 family of heterodimeric cytokines that also includes IL-12, IL-23, and IL-35, IL-27 is formed by the association of an IL-27-p28 subunit (also known as IL-30) with the Epstein-Barr Virus (EBV) -induced Gene 3 (EBI3) subunit (also known as IL-27B) . Expressed by antigen-presenting cells (APCs) in the early phases of antigen-mediated activation, IL-27 acts as a critical initiator of adaptive immune responses by promoting the rapid clonal expansion of naïve CD4+ T cells, IFN-gamma production, and Th1 polarization. IL-27 elicits its effects through receptor complexes IL-27R (also known as TCCR/WSX-1) and gp130, a receptor shared by IL-6. Mainly expressed in monocytes, endothelial cells, and dendritic cells, IL-27 plays an important role alongside IL-6 in the regulation of inflammation and autoimmunity; directly antagonizing IL-6’s stimulation of CD4+ T cell proliferation and Th17 differentiation. Recombinant Human IL-27 produced from HEK293 cells is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa.

Interleukin-27, Interleukin-27 subunit alpha, IL-27-A, Interleukin-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, p28, Interleukin-30, IL30

246778/10148

Q8NEV9/Q14213

NP_663634.2/NP_005746.2

NM_145659.3/NM_005755.2

Human

Mouse, Human

P28 subunit: FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP EBI3 subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

< 0.1 ng/µg of protein (< 1EU/µg)

> 90% by SDS-PAGE & HPLC analyses

Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml.

424

Lyophilized

47.8 kDa

HEK 293 cells