IL-27 Antilichaam

IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.

246778

Q8NEV9

NP_663634

Human, Mouse

3799

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar IL-27 expression profile in human and mouse cell lines detected by two independent anti-IL-27 antibodies that recognize different epitopes, 3797 against N-terminus domain and 3799 against central domain. IL-27 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3797 3799 Recombinant Protein Test (Figure 3) shows that anti-IL-27 antibody (3799) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 3799 antigenic peptide. Recombinant Protein Test

Immunology, Cancer

IL-27 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1288 - A20 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Pig: (92%), Rat: (79%)

IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

IL-27 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human IL-27 has one isoform (243aa, 28kD) . Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD) . 3797 can detect human, mouse and rat IL-27.

IL27

Cat. No. 17-207 - EL4 Cell Slide

Cat. No. 1201 - HeLa Cell Lysate

Cat. No. 1205 - Jurkat Cell Lysate

Cat. No. 1220 - SK-N-SH Cell Lysate

Interleukin 27

Homo sapiens

Pfanz et al. Immunity 2002; 16:779-90.

Pfanz et al. J. Immunol. 2004; 172:2225-31.

Hisada et al. Cancer Res. 2004; 64:1152-6.

IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha

ELISA, WB

28416913

Liquid