IL12B & IL23A Proteïne (C-His, Mouse)

IL12B & IL23A

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 95 % as determined by SDS-PAGE, ≥ 95 % as determined by SEC-HPLC, ≥ 95% as determined by SEC-MALS (Routinely tested) .

1.Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) (Cat: 13840-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse IL-23 Heterodimer Protein (His Tag) (Cat: CT140-M08H), the EC50 is 11-33 ng/mL. 2.Immobilized Recombinant Mouse IL-23 Heterodimer Protein (His Tag) (Cat: CT140-M08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL12RB1 Protein (ECD, Fc Tag) (Cat: 11674-H02H), the EC50 is 25-80 ng/mL (Routinely tested) . 3.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT140-M08H) with an affinity constant of 19.1 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Rat IL-23 Receptor Protein, hFc Tag (Cat. No. 80395-R02H) on proA Biosensor, can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT140-M08H) with an affinity constant of 18.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.01-0.2 ng/mL (QC tested) . 6.Loaded Recombinant Mouse IL-23 Receptor Protein, Fc Tag (Cat. No. 51293-M02H) on proA Biosensor, can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT140-M08H) with an affinity constant of 20.700 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Recombinant Rat IL-23 Receptor Protein, hFc Tag (Cat. No. 80395-R02H) captured on Protein A chip can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT140-M08H) with an affinity constant of 1.570 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Met1-Ser335 & Met1-Ala196

The recombinant heterodimer of Mouse IL12B & IL23A comprises (313+186) amino acids and has a calculated molecular mass of (35.8+21.11) KDa. The apparent molecular mass of Mouse IL12B & IL23A heterodimer is approximately 43.9、41.4、26.9 and 23.6 KDa respectively in SDS-PAGE. The molecular weight of this protein is around 62.2 kDa verified by SEC-MALS (Routinely tested) .

Mouse