IMPACT cDNA

IMPACT belongs to the IMPACT family and contains 1 RWD domain. It is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.

RWDD5

18q11.2-q12.1

Human

PATGen (puc19-derived cloning vector)

ATGGCTGAGGGGGACGCAGGGAGCGACCAGAGGCAGAATGAGGAAATTGAAGCAATGGCAGCCATTTATGGCGAGGAGTGGTGTGTCATTGATGACTGTGCCAAAATATTTTGTATTAGAATTAGCGACGATATAGATGACCCCAAATGGACACTTTGCTTGCAGGTGATGCTGCCGAATGAATACCCAGGTACAGCTCCACCTATCTACCAGTTGAATGCTCCTTGGCTTAAAGGGCAAGAACGTGCGGATTTATCAAATAGCCTTGAGGAAATATATATTCAGAATATCGGTGAAAGTATTCTTTACCTGTGGGTGGAGAAAATAAGAGATGTTCTTATACAAAAATCTCAGATGACAGAACCAGGCCCAGATGTAAAGAAGAAAACTGAAGAGGAAGATGTTGAATGTGAAGATGATCTCATTTTAGCATGTCAGCCGGAAAGTTCGGTTAAAGCATTGGATTTTGATATCAGTGAAACTCGGACAGAAGTAGAAGTAGAAGAATTACCTCCGATTGATCATGGCATTCCTATTACAGACCGAAGAAGTACTTTTCAGGCACACTTGGCTCCAGTGGTTTGTCCCAAACAGGTGAAAATGGTTCTTTCCAAATTGTATGAGAATAAGAAAATAGCTAGTGCCACCCACAACATCTATGCCTACAGAATATATTGTGAGGATAAACAGACCTTCTTACAGGATTGTGAGGATGATGGGGAAACAGCAGCTGGTGGGCGTCTTCTTCATCTCATGGAGATTTTGAATGTGAAGAATGTCATGGTGGTAGTATCACGCTGGTATGGAGGGATTCTGCTAGGACCAGATCGCTTTAAACATATCAACAACTGTGCCAGAAACATACTAGTGGAAAAGAACTACACAAATTCACCTGAGGAGTCATCTAAGGCTTTGGGAAAGAACAAAAAAGTAAGAAAAGACAAGAAGAGGAATGAACATTAA

IMPACT, RWDD5, IMPACT, ATGD0452-10 µg, ATGD0452-20 µg, ATGD0452-50 µg, ATGD0452-100 µg, ATGD0452-250 µg, ATGD0452-500 µg, ATGD0452-1 mg, ATGD0452-10, ATGD0452-20, ATGD0452-50, ATGD0452-100, ATGD0452-250, ATGD0452-500, ATGD0452-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

615319

NP_060909.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_018439.3

963bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.