Influenza A H1N1 Hemagglutinine antilichaam

ELISA: H1N1/HA1 Antibody (1 µg/mL) specifically recognizes H1N1 recombinant protein, but not interacted H3N2 and H5N1 recombinant protein in ELISA.{ATGA0221-Addpic.jpg}

Influenza virus, an enveloped virus of the Orthomyxoviridae family, has a unique capacity for genetic variation that is based in two molecular features of the virus family. First of all, the surface proteins of the virus are highly variable, able to mutate up to 50% of their amino acid sequence and still perform their functions in infection. Secondly, the viral genome is segmented, with eight RNA segments that are genetically independent of one another. In a mixed infection of different influenza genotypes, these segments can almost randomly reassort resulting in hybrid genotypes with some segments derived from one virus strain, while the other segments are derived from a second strain.

Hemagglutinin, Influenza A virus (A/New York/3571/2009 H1N1) hemagglutinin, HA

Influenza A-H1N1, Influenza A

Influenza A

Recombinant Influenza A-H1N1/HA1 (18-344aa) purified from Baculovirus

AT1G7

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

H1N1, HA1, Influenza, hemagglutinin, Influenza A virus (A/New York/3571/2009 H1N1) hemagglutinin, HA, ATGA0221-10 µg, ATGA0221-20 µg, ATGA0221-50 µg, ATGA0221-100 µg, ATGA0221-250 µg, ATGA0221-500 µg, ATGA0221-1 mg, ATGA0221-10, ATGA0221-20, ATGA0221-50, ATGA0221-100, ATGA0221-250, ATGA0221-500, ATGA0221-1

Kilbourne ED: Influenza pandemics of the 20th century. Emerg Infect Dis 2006,12:9-14. ; ; Russell RJ, et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (46) : 17736-41.; ; AJ. Hay, et al. Philos. Trans. R. Soc. Lond. B Biol. Sci. 356, 1861 (2001) .;

Sakamoto H, et al. A Novel Optical Biosensing System Using Mach-Zehnder-Type Optical Waveguide for Influenza Virus Detection. (Appl Biochem Biotechnol. 2016) {https://pubmed.ncbi.nlm.nih.gov/26498024/}; ; Shim DH, et al. Development of a HA1-specific enzyme-linked immunosorbent assay against pandemic influenza virus A H1N1. (Clin Exp Vaccine Res. 2019) {https://pubmed.ncbi.nlm.nih.gov/30775353/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Other

ACV04238.1

C7RYS4

The recombinant Influenza A-H1N1/HA1 proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Influenza A-H1N1/HA1 antibody (1:500) . (1:1000), (1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.