Influenza A H5N1 Hemagglutinine antilichaam

H5N1 is a subtype of the species Influenza A virus of the Influenzavirus A genus of the Orthomyxoviridae family. consists of single-stranded eight-segment negative-sense genomic RNAs, helical viral ribonucleoprotein (RNP) complexes (RNA segments NP, PB2, PB1 and PA), three viral envelope proteins (hemagglutinin [HA], neuraminidase [NA], and M2 ion channel), and a maxtir (M1) protein. Influenza A viruses are further classified into 16 HA (H1-H16) and 9 NA (N1-N9) serotypes based on the antigenic characteristics of HA and NA envelope glycoproteins. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.

Hemagglutinin, Influenza A virus (A/Vietnam/HN31242/2007H5N1) haemagglutinin

Human

Influenza A

Recombinant Influenza A H5N1/HA1 (17-338aa) purified from Baculovirus

AT2B7

Unconjugated

ELISA, WB

1 mg/mL (determined by BCA assay)

H5N1, HA1, ATGA0522-10 µg, ATGA0522-20 µg, ATGA0522-50 µg, ATGA0522-100 µg, ATGA0522-250 µg, ATGA0522-500 µg, ATGA0522-1 mg, ATGA0522-10, ATGA0522-20, ATGA0522-50, ATGA0522-100, ATGA0522-250, ATGA0522-500, ATGA0522-1

Song D., et al. (2008) Emerg. Infect. Dis. 14:741-746; ; Li S., et al. (2010) Infect. Genet. Evol. 10:1286-1288; ; Horimoto T, Kawaoka Y (2005) Influenza: lessons from past pandemics, warnings from current incidents. Nat Rev Microbiol 3: 591-600.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Virus

ABY19417

A9YU04

Recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Influenza A H5N1/HA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Influenza A H1N1 protein; ; Lane 2.: Recombinant Influenza A H3N2 protein; ; Lane 3.: Recombinant Influenza A H5N1/HA1 protein