Interferonregulatiefactor 5 Antilichaam / IRF5

Interferon regulatory factor 5, also called IRF5 or SLEB10, is a protein that in humans is encoded by the IRF5 gene. IRF5 gene is mapped to 7q32.1. This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. This gene is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection.

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q13568

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids R98-A477) was used as the immunogen for the Interferon regulatory factor 5 antibody.

WB, IF, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Interferon regulatory factor 5 antibody is available for research use only.

After reconstitution, the Interferon regulatory factor 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Interferon regulatory factor 5 antibody should be determined by the researcher.

Nuclear, cytoplasmic

Immunofluorescent staining of FFPE human A549 cells with Interferon regulatory factor 5 antibody (red) and Phalloidin-AF488 (green) . HIER: steam section in pH6 citrate buffer for 20 min.