IRAK4 Antilichaam

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

Q9NWZ3

Human

A portion of amino acids 5-36 from the human protein was used as the immunogen for this IRAK4 antibody.

WB, IHC, ELISA

Antigen affinity

Antigen affinity purified

This IRAK4 antibody is available for research use only.

Aliquot the IRAK4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

Titration of the IRAK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Bovine, Mouse

Western blot analysis of IRAK4 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the IRAK4 gene. Predicted molecular weight 52/37 kDa (isoforms 1/2) .