IRF-3 (fosfo Ser386) Antilichaam

Key transcriptional regulator of type I interferon (IFN) -dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

3661

Q14653

NP_001184052.1

Human

IRF-3 (phospho Ser386) antibody was raised against a peptide sequence around phosphorylation site of Serine 386 (A-S-S (p) -L-E) derived from Human IRF-3.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

55 kDa

IgG

The antibody detects endogenous levels of IRF-3 only when phosphorylated at serine 386.

Western Blot: 1:500~1:1000

IRF3

Interferon regulatory factor 3

Homo sapiens

Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661 (1995) .

The MGC Project Team; Genome Res. 14:2121-2127 (2004) .

Bellingham J., Ann. Hum. Genet. 62:231-234 (1998) .

IRF3, Interferon regulatory factor 3

WB

4504725

Liquid