IRS-1 Antilichaam

IRS-1 Antibody: Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL) -4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR) . TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.

3667

P35568

P35568

Human, Mouse

Unconjugated

Primary Antibodies

Signal Transduction

IRS-1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1216 - PC-3 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat: (80%)

IRS-1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

IRS-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

IRS1

Cat. No. 1286 - P815 Cell Lysate

Cat. No. 17-206 - P815 Cell Slide

Insulin receptor substrate 1

Homo sapiens

Giovannone B, Scaldaferri ML, Federici M, et al. Insulin receptor substrate (IRS) transduction system: distinct and overlapping signaling potential. Diabetes Metab. Res. Rev. 2000; 16:434-41.

Waters SB and Pessin JE. Insulin receptor substrate 1 and 2 (IRS1 and IRS2) : what a tangled web we weave. Trends in Cell Biol. 1996; 6:1-4.

Ozes ON, Akca H, Mayo LD, et al. A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1. Proc. Natl. Acad. Sci. USA 2001; 98:4640-5.

Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.

IRS-1 Antibody: HIRS-1, Insulin receptor substrate 1, IRS-1

ELISA, WB, ICC, IF

547738

Liquid