IRX1 Antilichaam / Iroquois-klasse homeodomaine eiwit

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

P78414

Human, Mouse, Rat

E.coli-derived human IRX1 recombinant protein (Position: Q95-A480) was used as the immunogen for the IRX1 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This IRX1 antibody is available for research use only.

After reconstitution, the IRX1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the IRX1 antibody should be determined by the researcher.

Western blot analysis of IRX1 using anti-IRX1 antibody. Lane 1: human U251 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse LLC tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRX1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for IRX1 at approximately 50 kDa. The expected band size for IRX1 is at 50 kDa.